Journal: Bioresources and Bioprocessing

Article Title: Box-Behnken optimized copper oxide nanoparticles from Thymus vulgaris potentiate efficacy against multidrug-resistant bacterial pathogens and exhibit anticancer activity

doi: 10.1186/s40643-026-01008-5

Figure Lengend Snippet: Comprehensive Evaluation of TE-CuONPs Anticancer Activity and Apoptotic Mechanism in MCF-7 Breast Cancer Cells. Concentration-dependent cytotoxicity and mechanistic analysis. a , b MTT dose–response curves against MCF-7 breast cancer cells and normal human skin fibroblasts (HSF). c , d Representative phase-contrast micrographs (200 × magnification) of MCF-7 cells after 24 h treatment at respective IC₅₀ concentrations, showing c minimal morphological changes with TE treatment versus d characteristic apoptotic features with TE-CuONPs treatment, including cell shrinkage (red arrows), membrane blebbing (yellow arrows), chromatin condensation, and cellular detachment. e Flow cytometric scatter plots (FSC-H vs. SSC-H) demonstrating consistent cell populations across treatments (10,000 events gated per sample). ( f ) Schematic proposal for anticancer mechanisms. g Annexin V-FITC/propidium iodide (PI) dual-staining flow cytometric analysis

Article Snippet: MCF-7 breast adenocarcinoma cells (ATCC HTB-22) and normal human skin fibroblasts (HSF, CRL-2522) were obtained from Nawah Scientific (Cairo, Egypt) and cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 5% CO2.

Techniques: Activity Assay, Concentration Assay, Membrane, Staining

Journal: NPJ Science of Food

Article Title: From rosemary and coffee to bioactive nanovesicles: exploring new frontiers in food functional ingredients

doi: 10.1038/s41538-026-00723-9

Figure Lengend Snippet: Evaluation of the effects of the RVs and CVs on H 2 O 2 -induced reactive oxygen species (ROS) and MDA production levels in fibroblast BJ-5TA cells ( A , B ) and differentiated C2C12 ( C , D ) cells. The data points represent the averages ±SD of three independent experiments in duplicate. All data sets were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Different lowercase letters indicate a significant difference ( p < 0.05) between different treatments. Control: untreated cells. RVs: rosemary vesicles; CVs: coffee vesicles. 7.8 ×10 6 vesicles/mL and 39 ×10 6 vesicles/mL correspond to 0.1 and 0.5 mg nanovesicles/mL.

Article Snippet: hTERT-immortalized human skin fibroblasts BJ-5TA cell line was bought from ATCC (ATCC (CRL-4001 TM), from LGC Standards, Milan, Italy), cultured in DMEM high glucose and Medium 199 (4:1 ratio) with stable L-glutamine supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin and incubated at 37 °C under a 5% CO 2 atmosphere.

Techniques: Control